Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35)
CAT#: NS-080CN
The anti-SIRPα antibody clone 29-AM3-35 targets to SIRPα. The antibody was screened from a phage display library. It can be used for treating studies of hematological cancer, particularly leukemia.
Published Data
Gene Expression
Tested Data
Inhib
Figure 1 The binding inhibition by three anti-SIRPa antibody format compounds (AM3-35, AM4-5 and AM4-1) of binding between CD47-Fc and hSIRPα V2 expressed on mouse macrophages.
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Normal Tissue
Figure 1 Cerebral cortex
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_7_3.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (fHDF/TERT166, RPTEC TERT1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198053-SIRPA
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SDS-PAGE
Figure 1 Anti-SIRPA Recombinant Antibody (NS-080CN) in SDS-PAGE
SDS-PAGE analysis of NS-080CN in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
WB
Figure 2 Anti-SIRPA Recombinant Antibody (NS-080CN) in WB
Western blot analysis of NS-080CN was performed by loading human SIRPA protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with NS-080CN and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
DB
Figure 3 Anti-SIRPA Recombinant Antibody (NS-080CN) in DB
Dot Blot analysis of NS-080CN was performed by coating with human SIRPA protein (His Tag).
NS-080CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
ELISA
Figure 4 Anti-SIRPA Recombinant Antibody (NS-080CN) in ELISA
ELISA analysis of NS-080CN was performed by coating with human SIRPA protein (His Tag). Then blocked with BSA and incubated with NS-080CN. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Human SIRPA
- Species Reactivity
- Human
- Clone
- 29-AM3-35
- Applications
- ELISA, Inhib, FuncS
- Related Disease
- Hematological cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Inhibition, Functional Assay.
Target
- Alternative Names
- SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs
- Gene ID
- 140885
- UniProt ID
- P78324
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 29-AM3-35"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1201-VHH | Recombinant Anti-Mouse Sirpa VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-453CT | Human Anti-SIRPA Recombinant Antibody (TAB-453CT) | Cyt, IF, FC | Chimeric (mouse/human) IgG4 |
| TAB-453CT-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (TAB-453CT-S(P)) | WB, ELISA | Human scFv |
| TAB-453CT-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (TAB-453CT-F(E)) | WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-454CT | Anti-Human SIRPA Recombinant Antibody (AM4-1) | ELISA | Human antibody |
| TAB-454CT-S(P) | Anti-Human SIRPA Recombinant Antibody scFv Fragment (AM4-1) | ELISA | Human antibody |
| TAB-454CT-F(E) | Anti-Human SIRPA Recombinant Antibody Fab Fragment (AM4-1) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0465MZ | Chicken Anti-PTPNS1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1970CQ | Recombinant Rat Anti-Sirpa Antibody (P84) | FC, BL, IF, IHC, IP | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1971CQ | Recombinant Mouse Anti-Sirpa Antibody (CBL650) | WB, FC, CyTOF, Neut | IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4429 | Hi-Affi™ Recombinant Rabbit Anti-SIRPA Monoclonal Antibody (SI522DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0023-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0024-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4); scFv Fragment | ELISA | Humanized scFv |
| HPAB-N0386-YC-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0386-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-N0387-YC-S(P) | Mouse Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0387-YC-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-N0388-YC-S(P) | Mouse Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0388-YC-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0023-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0024-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0025-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-5); Fab Fragment | ELISA | Humanized Fab |
| HPAB-N0386-YC-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-N0386-YC-F(E)) | ELISA, FC | Human Fab |
| HPAB-2559LY-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-2559LY-F(E)) | Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-080CN-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); Fab Fragment | ELISA | Human Fab |
| NS-080CN-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0078 | Human Anti-SIRPA Recombinant Antibody (PABJ-0078) | ELISA | Human IgG |
| PABJ-0084 | Human Anti-SIRPA Recombinant Antibody (PABJ-0084) | ELISA, Block | Human IgG |
| VS3-FY1336 | Recombinant Rabbit Anti-SIRPA Antibody (clone R04-7G6) | WB | Rabbit IgG |
| VS3-FY1337 | Recombinant Rabbit Anti-SIRPA Antibody (clone R08-1G4) | WB, ICC, IF | Rabbit IgG |
| VS-0224-XY114 | Mouse Anti-SIRPA Recombinant Antibody (VS-0224-XY114) | ELISA, WB, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1462 | AbPlus™ Anti-SIRPA Magnetic Beads (VS-0724-YC1462) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY86 | Human Anti-SIRPA (clone 29-AM3-35) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC143 | Recombinant Anti-SIRPA Vesicular Antibody, EV Displayed (VS-0425-YC143) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6598 | Anti-SIRPA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6599 | Anti-Mouse SIRPA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6600 | Anti-Rat SIRPA Immunohistochemistry Kit | IHC |
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